AIMS: The aims of the present study were to present the anthropometric characteristics (body weight and height) of the students of the health studies, to examine the frequency of negative health behaviors (alcohol, cigarettes and coffee consumptions) and to examine the predictive significance of personality traits and physical activity to depression, anxiety and stress in 3rd year undergraduate students. METHODS: The measuring instruments were as follows: a general data questionnaire containing sociodemographic characteristics, data on body weight, height and BMI index, and questions on life habits (alcohol and cigarette consumption); Depression, Anxiety and Stress Scale (DASS); BFI questionnaire; International Physical Activity Questionnaire (IPAQ). RESULTS: The results of the research indicated that personality trait Neuroticism had an influence on the occurrence of depression (t = 4.48; p <0.001), anxiety (t = 4.31; p <0.001) and stress (t = 5.35; p <0.001). Personality trait Openness to experiences contributed to higher student stress. A sedentary lifestyle affected the occurrence of anxiety (t = 2.31; p = 0.024). The level of depression in non-smokers was 0.29 points higher than in smokers (p> 0.05). Students who do not consume alcohol had a higher level of depression by 0.61 points (t = 0.81; p = 0.209), anxiety by 1.48 points (t = 1.63; p = 0.053) and stress by 1.21. point (t = 1.19; p = 0.120) in relation to students who do not consume alcohol. The students who do not drink coffee had a higher level of depression (by 0.84 points), anxiety (by 1.13 points) and stress (by 1.11 points) compared to students who consume coffee (p> 0.05). CONCLUSIONS: According to the obtained results, we can conclude that Neuroticism and physical inactivity affect the occurrence of anxiety while Openness to experiences contributed to higher stress in students.