AIM: The main goal of this study was to investigate and analyze the nature of the relationship between night work and stress and consequent benzodiazepine consumption. SUBJECTS AND METHODS: The study involved 609 nurses, 230 of whom consumed benzodiazepines. The questionnaire was created in a Google form and was distributed via social networks (WhatsApp, Facebook). The questionnaire consisted of 3 parts; sociodemographic part, Questionnaire for testing the use of benzodiazepines among nurses and Self-assessment questionnaire for determining dependence on benzodiazepines - Bendep-SRQ. RESULTS: Consumption of benzodiazepines is highest in bachelors of nursing, followed by masters, while high school graduates achieve the lowest rank. Most nurses feel that their work is physically and mentally exhausting, but they do not think that medication helps them forget about problems at work. They mostly do not take medicine at work from medicine cabinets or after a night shift. They do not think that the medicine is life-threatening, nor does it help them to withstand the effort at work more easily. CONCLUSION: Night work is not positively related to benzodiazepine consumption among nurses, while stress levels are.