Objective: To analyze the frequency of strokes at the Institute of Emergency Medicine of Split-Dalmatia County before, during, and after the COVID-19 pandemic, and to determine the differences in stroke frequency based on time periods, gender, age, and the most prevalent period within the calendar year. Methods: This cross-sectional study utilized data from the e-Hitna system of the Institute of Emergency Medicine of Split-Dalmatia County. The inclusion criteria were patients admitted at the Institute of Emergency Medicine of SplitDalmatia County with a diagnosis of stroke in the time period from January 1, 2019, to December 31, 2022. The patients were categorized by age group, gender, month, and season of stroke occurrence. The difference in stroke incidence before, during, or after the COVID-19 pandemic was calculated using the χ2 test, and the level of statistical significance was set at p<0.05. Findings: This study included a total of 3292 participants. In the year 2019, the number of affected individuals was 852, in 2020 it was 760, in 2021 it was 822, and in the year 2022, the number of affected individuals was 858 (χ2 = 7.034, p = 0.071). Throughout all analyzed years, the affected individuals were most commonly female and between 61-80 years of age. Conclusions: The frequency of strokes is lower during the COVID-19 pandemic compared to the pre-pandemic and post-pandemic years (χ2 = 7.034, p = 0.071). There is no difference in gender and age group distribution among individuals affected by stroke before, during, and after the COVID-19 pandemic.