Objective: The aim of this study was to examine changes in biochemical and hematological parameters that occur during storage of platelet concentrates. Materials and Methods: We analyzed ten platelet concentrates on the first day of production, as well as ten concentrate on the sixth day from the date of production. In these samples biochemical parameters (sodium, potassium and pH) were analyzed by the analyzer RAPIDlab 1265, Siemens. Hematological parameters, leukocyte count, platelet count and platelet indices (MPV, PDW and PCT) were analyzed on a hematology analyzer Advia 2120, Siemens. Statistical analysis was done using Microsoft Excel 2010th. The arithmetic mean, standard deviation, median, the maximum and minimum mean were calculated for the obtained values. Results: The results showed minor changes in the parameters examined in platelet concentrates first and sixth days of storage. Value potassium sixth days of production increased by 0.15 mmol/L, sodium level decreased by 0.1 mmol/L, mean platelet volume decreased by 0.1 fl and pH decreased by 0,14. Conclusion: The differences between the studied parameters (Na, K, pH, leukocytes, platelets and platelet index) in the platelet concentrate on the first day of production, and on the sixth day of production are minimal. Results of this study indicates that the examined parameters are still within acceptable limits at the end of the shelf life. This study have an incentive to explore some other parameters in order to study the quality and function of platelet concentrates to extend shelf life.