Objective: The aim of this study is to compare the titer of anti-erythrocyte antibodies in the serum of immunized pregnant women using the tube method and micro methods (Ortho and Diamed). The Ortho and Diamed microhemagglutination methods are more sensitive than the tube method, and therefore, the antibody titer will be higher in the microhemagglutination methods compared to the classical tube method. Materials and methods: 7 patients from the UHC Split with positive indirect antiglobulin test (IAT) were selected and sent for antibody titer determination. A total of 9 samples were processed from the 7 patients. The antibody titer was determined using the conventional tube method and the Ortho and Diamed micro methods. Results: The majority of patients showed critical and higher antibody titers. Micro methods demonstrated higher antibody titers compared to the tube method. Results are presented as the last positive titer and the last positive tube. Conclusion: The micro methods (Ortho and Diamed) show higher antibody titers. This difference is consistent across all samples, confirming the sensitivity of the micro methods. The tube method can differentiate between complete (IgM) and incomplete (IgG) antibodies, whereas micro methods exclusively determine the titer of IgG antibodies. The advantages of micro methods, such as their sensitivity and precision, can reduce the number of false-negative results. However, it is important to understand the implications of higher titers presented by the micro methods. Without clear reference intervals and standardized protocols, there is a risk of overdiagnosis and overtreatment.