Research objectives: The aim of this research is to determine if there are differences of opinion between undergraduate and graduate students, of the University Department of Health Studies in Split, about nursing as a profession. Furthermore, the aim is to show the differences in the attitudes of the students in the first and last years of nursing studies, and to determine whether the students' attitudes at the beginning of their studies are more positive / optimistic than the attitudes of the students at the end of their education. Methods: The research was conducted on a suitable sample of undergraduate and graduate students of nursing, at the University Department of Health Studies in Split. The validated questionnaire “Attitude toward Nursing Questionnaire” was used to collect the data and the respondents also provided the following data: gender, age, year of study, qualification, place of employment, years of work experience and marital status. The surveys were distributed and filled in electronically. Results: This research showed the attitudes of undergraduate and graduate nursing students, at the Department of Health Studies in Split, about nursing as a profession. Analyzes of variance gave results indicating statistically significant differences among different years of study about attitudes toward nurses' roles, tasks, and characteristics (F (5, 130) = 3.784, p = 0.003). The differences found are the mean effect sizes (ω2 = 0.093). Analysis of variance did not find statistically significant differences in professionalism, values and stereotypes about nursing in the society (p> 0.05). Conclusions: The views of undergraduate and graduate students on nursing as a profession are no different. 3rd year undergraduate students are more positive about the roles, tasks and characteristics of nurses than 1st year undergraduate students.