A retrospective study included 205 patients treated at the Department of Gynecology and Obstetrics, University Hospital Split during 2023,analyzing their age, assisted reproduction methods, and hormonal status. The patients were divided into three groups according to the treatment method: in vitro fertilization (IVF) or intracytoplasmic sperm injection (ICSI) (131 patients), frozen embryo transfer (FET) (48 patients), andartificial insemination by husband (AIH) (27 patients). The largest number of patients was in the IVF/ICSI group, with the youngest being 24 years old and the oldest 44 years old. In the FETgroup, the age of patients ranged from 28 to 41 years, while in the AIH group, it was between 27 and 42 years. Older age of the patients wasassociated with lower quality of oocytes, reducing the chances of pregnancy success, as confirmed in this study. The analysis of hormonal profiles showed that the average values of FSH, LH, and DHEA were within the reference values. The hormonesE2, PRL, and progesterone showed elevated values, while SHBG, vitamin D, and ferritin were within the reference values, except for SHBG inpatients treated with the FET method. The highest values were for the hormones E2 (34534 IU/L), PRL (3280 mU/L), and progesterone (70.80nmol/L), which is significantly above the reference values. Most patients had extremely low values of the Anti-Müllerian hormone (AMH). The study results emphasize the need for an individualized approach to infertility treatment, considering the hormonal profiles of women before and after treatment. Educating women about the causes of infertility and timely recognition of problems can contribute to better treatment outcomes.