AIM: The aim of this study was to examine the knowledge and attitudes of students of the University Department for Health Studies about organ transplantation regarding gender, age and study direction. METHODS: The measuring instrument used was a newly constructed Questionnaire of the knowledge and attitudes of students of the University Department for Health Studies in Split about organ transplantation, which was solved by a total of 210 students from the five study programs of the Undergraduate University Study and three study programs of the Graduate University Study. The study included 24 male and 186 female examinees. RESULTS: The results showed statistically significant differences in student knowledge only in the age group. A statistically significant difference in student attitude was not established, however, without any doubt, students have positive attitude towards organ donation. High percentage of students did not show knowledge about brain death confirmation, they expressed concern about possible manipulation and wish for education. CONCLUSION: According to the results we can conclude that the knowledge of students about organ donation is at higher level in older students. Although student attitudes towards organ donation are positive, additional education about this subject is required.