Introduction: Vaccination is the most prominent medical success of the 20th century, saving more lives than any other form of medical intervention. Public health programs of mass vaccination have marked an improvement in disease prevention and control. Aim: to determine Nursing and Midwifery students' stance on childhood vaccination. Hypothesis: Over 85% of Nursing and Midwifery students will hold a positive attitude towards childhood vaccination, regardless of place of study. Sample: Research participants are second and third year Nursery and Midwifery students in Croatia. Research was conducted through an online survey in June and July of 2018. A hundred and ninety-three participants include thirty-four Midwifery students and a hundred and fifty-three Nursery students. Participation is entirely voluntary and anonymous. Research method: Online survey numbering nineteen questions contained four questions concerning demographic information, and fifteen questions regarding students' opinions on vaccination. Participants were asked to offer their answers using a Likert scale ranging fom 1 to 5. The survey was shared on social media websites such as facebook.com, via websites of the Alumni from the University department of Health Studies, Croatian Chamber of Midwives, and the Nurse and Medical Technician Initiative. This survey was also shared on private online profiles, as well as though social mobile applications Whatsapp and Viber. Statistical analysis: Data collected through the online questionnaire was imported into a Microsoft Excel document according to a code plan previously prepared. Descriptive statistics were used for data analysis. To determine statistical significance, a t-test was used. Results: A total of 85.5% of participants agree or strongly agree with a statement that says vaccination is the most efficient method of disease prevention. 81.8% of participants consider potential risks negligible overall. 72.7% thinks that all health professionals should promote vaccination as the optimal preventive measure to protect against infectious diseases. 88.6% would have their children vaccinated according to the vaccination schedule. 85.5% of all participants deem a legal obligation to vaccinate children necessary. Children whose parents refused to have them vaccinated without valid reason should not be allowed kindergarten enrollment, according to 71.5% of participants, while 85.8% thinks that educating the public about the benefits of vaccination would reduce the reluctance and negative attitude towards vaccination. Conclusion: Nursery and Midwifery students hold positive opinions on childhood vaccination (85.5%). 88.6% of students questioned would vaccinate their children according to schedule. Results show that the majority of participants hold no opinion on the representation of the subject in textbooks; those who strongly disagree that the subject is represented enough number M-5.2, believing that educating the public about benefits of vaccination would reduce the number of people holding negative opinions on the subject. Participants deem it necessary to revise the curriculum and to add emphasis on the subject of vaccination and its benefits for the entire population. Health education is a vital function of both nursery and midwifery, therefore in formal education of future students as well as in lifelong education after graduation, it is highly necessary that thorough education on vaccination be implemented. This way, we would have public health education and enlightenment that are based on actual proof.