Purpose: The aim of this study is to investigate job satisfaction of the nurses employed in the operating rooms and surgical wards at the Department of Surgery of the University Hospital of Split, and to determine whether there is a difference in the job satisfaction of the employees in the operating room with respect to the surgical Wards. Respondents working in the operating rooms are assumed to have a lower degree of job satisfaction than respondents employed in the surgical Wards. Respondents and Methods: A total of 82 nurses of all educational levels participated in the study. The study was conducted among two groups of respondents. The first group of respondents consists of 47 nurses employed in the inpatient unit - Department of Surgery, University Hospital of Split. The second group of respondents consists of 35 nurses employed in the operating rooms of the Surgery Clinic of the University Hospital of Split. All respondents are female. Data have been collected by usage of an anonymous questionnaire. Results: Based on the analysis of the results obtained, it can be established in total that nurses are dissatisfied with their job regardless of their place of work. However, it can be firmly concluded that the assumption by which was expected that nurses employed in the operating rooms would express a lower degree of job satisfaction did not confirm the results of the study. A higher level of dissatisfaction has been expressed by the nurses employed in the surgical ward compared to the nurses employed in the operating rooms. The highest level of satisfaction in both groups of respondents has been expressed in the field of communication. Conclusion: Satisfaction is a key factor that motivates an employee to work more productively and to achieve personal goals as well as organization's goals and objectives. Motivation needs to be recognized and encouraged. Trained leaders are a necessity in order to help an individual to deliver results as expected. It should not be neglected that a nurse follows the life from the beginning to the death, therefore it is necessary to renew the affection for the nursing profession, to improve it for more satisfied nurses in the future.