Research objectives: The aim of this study is to investigate if there is a significant influence of shift work on sleep quality and sleep disturbances as well as attention disorders in hospital nurses. Another aim of the study is to investigate if this relationship is dependent on other contextual factors, such as the department on which a respondent is employed or years spent in shift work. This study also investigates the differences in circadian rhythms between shift and non.-shift workers. Methods: The research was conducted in KB Merkur on a pattern taken out of the nurse and medical technician population employed in the hospital. The aim of the research was to have greater representativeness by including respondents from different departments as well as of different age and work experience. Data was collected by a survey that contained questions regarding sleep quality, mindful attention awareness and morningness –eveningness. Results: This research has shown the influence of shift work on sleep quality, mindful attention and circadian rhythm of the respondents as well as the influence of contextual factors on these relationships. Conclusions: There are a number of important findings that can be stated from this study. The first one is that there is a significant difference in sleep quality between shift and non-shift workers, and non-shift workers have a better sleep quality. Besides that, there is a light and positive correlation between years of work experience and sleep quality in shift workers, while the same cannot be stated for non-shift workers where such a relationship does not exist. Also, the study showed that there is no significant difference in mindful attention awareness between shift and non-shift workers. There is also no statistically relevant correlation between years of work experience and mindful attention awareness in shift and non-shift workers alike. However, it is interesting to notice that the correlation coefficient, although not statistically significant is positive for non-shift, and negative for shift workers. The department on which a respondent is employed has not shown to be an important predictor for difference in sleep quality of mindful attention awareness. Circadian rhythm analysis has shown that non-shift workers are, on average, more morning types than shift workers and that there is a significant difference between circadian rhythms of shift and non-shift workers.