When we are discussing the protection of individuals and population from many infectious diseases, it is safe to say that nowadays vaccination against contagious diseases is the most successful and cost-effective, as well as being very often the only possible method of modern medicine to prevent it. The main objective of the research was to determine the attitudes and knowledge of nursing students at the University Department of Health Studies in Split and the nurses/technicians employed at Clinical hospital center Split towards the vaccination of children and to investigate their correlation with vocational education, work experience, program and year of study. The research and graduate thesis is based on an anonymous survey of students at the University Department of Health Studies in Split and the medical nurses/technicians employed at Clinical hospital center Split. The results of the study showed positive attitudes towards vaccination in all observed groups, 86.32% believe that vaccination is the most effective method of preventing contagious diseases. 75.62% of respondents believe that the beneficial effects of vaccination are significantly higher than the potential risks and 63.68% of respondents believe the vaccines are safe and well-controlled. 89,80% of the respondents would regularly vaccinate their child by the vaccination schedule. 64.18% of respondents believe that the topic of vaccination is not sufficiently represented in curriculum / planed educations of HKMS. The best knowledge in solving the test of knowledge has been shown by nurses with the bachelor´s degree. 51.49% of respondents use appropriate sources of vaccine information. Most respondents have a positive opinion about vaccination and understand the importance of high-quality continuous vaccination proliferation. Better education is needed, greater representation of the subject itself in the teaching plan, and encouragement to promote vaccination. Key words: vaccination, knowledge, attitude, nursing, student, nurse