Unhealthy nutrition and physical inactivity are leading global health risks for public health and cause many non-communicable chronic diseases today. The aim of this study was to determine differences in knowledge, attitudes and habits about physical activity and proper nutrition in students of the University Department of Health Studies in Split in terms of gender, age and study program. Method that was used is a newly constructed questionnaire that 228 of the students answered, from five undergraduate university study programs and from Graduate school. 202 female and 26 male students participated between 18 and 41 years of age. This research has shown, regarding the gender, that female students follow the recommended guidelines on proper nutrition and physical activity more than male students. Splitting the students into age groups, it has shown that older students have better attitudes and knowledge about proper nutrition and physical activity but also claim that they don’t have time for physical activity more than students in younger age groups so they don’t follow the guidelines as much, while students in younger age groups engage in physical activity more. There have also been differences determined between students in different study programs. Students in Medical Laboratory Diagnosis study program, Radiology Technology study program and Nursing smoke significantly less, while Physiotherapy students smoke the most. Students that engage in physical activity the least are Graduate students and Midwifery students, while Physiotherapy students engage the most. In contrast, Physiotherapy students also spend the most time sitting down daily, while Graduate students spend the least time sitting down. Significant differences between students of different genders, ages and study programs on attitudes and habits of proper nutrition and physical activity have been determined.