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undergraduate thesis
Instrumentalne tehnike u otkrivanju infekcije COVID-19
2022. urn:nbn:hr:176:950715
Labor, Pio
University of Split
University Department of Health Studies

Cite this document

Labor, P. (2022). Instrumentalne tehnike u otkrivanju infekcije COVID-19 (Undergraduate thesis). Split: University of Split. Retrieved from https://urn.nsk.hr/urn:nbn:hr:176:950715

Labor, Pio. "Instrumentalne tehnike u otkrivanju infekcije COVID-19." Undergraduate thesis, University of Split, 2022. https://urn.nsk.hr/urn:nbn:hr:176:950715

Labor, Pio. "Instrumentalne tehnike u otkrivanju infekcije COVID-19." Undergraduate thesis, University of Split, 2022. https://urn.nsk.hr/urn:nbn:hr:176:950715

Labor, P. (2022). 'Instrumentalne tehnike u otkrivanju infekcije COVID-19', Undergraduate thesis, University of Split, accessed 24 December 2024, https://urn.nsk.hr/urn:nbn:hr:176:950715

Labor P. Instrumentalne tehnike u otkrivanju infekcije COVID-19 [Undergraduate thesis]. Split: University of Split; 2022 [cited 2024 December 24] Available at: https://urn.nsk.hr/urn:nbn:hr:176:950715

P. Labor, "Instrumentalne tehnike u otkrivanju infekcije COVID-19", Undergraduate thesis, University of Split, Split, 2022. Available at: https://urn.nsk.hr/urn:nbn:hr:176:950715

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Metadata
TitleInstrumentalne tehnike u otkrivanju infekcije COVID-19
Title (english)Instrumental techniques in detecting COVID-19 infection
AuthorPio Labor
MentorSendi Kuret (mentor)
Committee memberEsma Čečuk-Jeličić (predsjednik povjerenstva)
Committee memberDavorka Sutlović (član povjerenstva)
Committee memberSendi Kuret (član povjerenstva)
GranterUniversity of Split
(University Department of Health Studies)
Split
Defense date and country2022-07-21, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Clinical Medical Sciences
Abstract
Cilj: Opisati instrumentalne tehnike i objasniti njihovu primjenjivost u otkrivanju COVID-19 infekcije. Materijal i metode: Opisan je postupak izvođenja RT-qPCR reakcije od izolacije RNA do detekcije kao i RT-LAMP metode. Prikladni materijal potrebni za postupke RT-qPCR-a i RT-LAMP-a su također opisani. Rezultati: Jačina fluorescencije PCR amplikona se predočava elektroferogramom na kojem se prikazuje na apscisi broj ciklusa reakcije, a na ordinati jačina fluorescencije. Ct vrijednost je obrnuto proporcionalna količini virusa mjerenog uzorka. Za RT-LAMP metodu će se na ekranu aparata pokazati pozitivan, negativan ili neispravan rezultat. Zaključak: RT-qPCR je zlatni standard u detekciji COVID-19 infekcija zbog svoje visoke preciznosti i osjetljivosti. RT-LAMP će se u bliskoj budućnosti više koristiti zbog brzine detekcije i jednostavnosti uporabe. Sekvenciranje je ključ u identificiranju različitih podtipova SARS-CoV-2.
Abstract (english)
Objectives: To describe instrumental techniques and explain their applicability in the detection of COVID-19 infection. Material and methods: The procedure for performing the RT-qPCR reaction from RNA isolation to detection is described, as well as the RT-LAMP method. Appropriate material required for RT-qPCR and RT-LAMP procedures are also described. Results: The fluorescence intensity of the PCR amplicon is represented by an electropherogram showing the number of reaction cycles on the abscissa and fluorescence intensity on the ordinate. The Ct value is inversely proportional to the amount of virus in the measured sample. For the RT-LAMP method, a positive, negative or invalid result will be displayed on the device screen. Conclusion: RT-qPCR is the gold standard in the detection of COVID-19 infections due to its high precision and sensitivity. RT-LAMP will be used more in the near future due to its speed of detection and ease of use. Sequencing is the key to identifying the different subtypes of SARS-CoV-2.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:176:950715
Study programmeTitle: Medical Laboratory Diagnostics (university/undergraduate)
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni prvostupnik/ prvostupnica (baccalaureus/ baccalaurea) medicinske laboratorijske dijagnostike (sveučilišni prvostupnik/ prvostupnica (baccalaureus/ baccalaurea) medicinske laboratorijske dijagnostike)
Type of resourceText
File originBorn digital
Access conditionsAccess restricted to students and staff of home institution
Terms of useLicence In copyright icon
Created on2022-07-21 08:19:06