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undergraduate thesis
Molekularna dijagnostika virusa SARS-CoV-2
2022. urn:nbn:hr:176:700298
Huzjak, Klara
University of Split
University Department of Health Studies

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Huzjak, K. (2022). Molekularna dijagnostika virusa SARS-CoV-2 (Undergraduate thesis). Retrieved from https://urn.nsk.hr/urn:nbn:hr:176:700298

Huzjak, Klara. "Molekularna dijagnostika virusa SARS-CoV-2." Undergraduate thesis, University of Split, University Department of Health Studies, 2022. https://urn.nsk.hr/urn:nbn:hr:176:700298

Huzjak, Klara. "Molekularna dijagnostika virusa SARS-CoV-2." Undergraduate thesis, University of Split, University Department of Health Studies, 2022. https://urn.nsk.hr/urn:nbn:hr:176:700298

Huzjak, K. (2022). 'Molekularna dijagnostika virusa SARS-CoV-2', Undergraduate thesis, University of Split, University Department of Health Studies, accessed 30 March 2023, https://urn.nsk.hr/urn:nbn:hr:176:700298

Huzjak K. Molekularna dijagnostika virusa SARS-CoV-2 [Undergraduate thesis]. Split: University of Split, University Department of Health Studies; 2022 [cited 2023 March 30] Available at: https://urn.nsk.hr/urn:nbn:hr:176:700298

K. Huzjak, "Molekularna dijagnostika virusa SARS-CoV-2", Undergraduate thesis, University of Split, University Department of Health Studies, Split, 2022. Available at: https://urn.nsk.hr/urn:nbn:hr:176:700298

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Metadata
TitleMolekularna dijagnostika virusa SARS-CoV-2
Title (english)Molecular diagnostics of SARS-CoV-2 virus
AuthorKlara Huzjak
MentorVanja Kaliterna (mentor)
Committee memberEsma Čečuk-Jeličić (predsjednik povjerenstva)
Committee memberKatarina Šiško Kraljević (član povjerenstva)
Committee memberVanja Kaliterna (član povjerenstva)
GranterUniversity of Split
University Department of Health Studies
Split
Defense date and country2022-07-21, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Clinical Medical Sciences
Abstract
Cilj rada: Cilj ovog rada bio je teorijski prikazati i razjasniti karakteristike i građu virusa SARS-CoV2, opisati trenutno dostupne dijagnostičke metode za analizu virusa i prikazati najbolju dijagnostičku tehniku, RT-PCR molekularnu metodu. Materijali i metode: U ovom radu opisan je postupak molekularne metode dijagnostike SARS-CoV-2 dijagnostike na BIO-RAD CFX96 RT- PCR uređaju uz korištenje reagensa LiliF Master Mix kit za pripremu reakcijske smjese, koji se koriste u Nastavnomzavodu za javno zdravstvo Splitsko-dalmatinske županije Rezultati: Svi uzorci moraju imati detektiranu IPC (Internu kontrolu) koja dokazuje da je postupak obrade uzorka dobro napravljen, te se može pristupiti interpretaciji nalaza. Pozitivan nalaz na virus SARS-CoV-2 označava prisutnost tri detektibilna gena: E,RdRp i N, dok za negativan rezultat geni nisu eksprimirani. Zaključak: Najbrža, najosjetljivija i najisplativija dijagnostička metoda za detekciju i kvantifikaciju novonastalog virusa jest RT-PCR
Abstract (english)
Objectives: The aim of thisstudy wasto theoretically present and clarify the characteristics and structureof the SARS-CoV-2 virus, to describe the currently available diagnostic methods for the analysis of the virus and to present the best diagnostic technique, the RT-PCR molecular method. Methods: This manuscript describes the procedure of the molecular method for SARS-CoV-2 detection on the BIO-RAD CFX96 RT-PCR instrument using LiliF Master Mix kit reagents for the preparation of the reaction mixture, which are used at the Teaching Public Health Institute of Split-Dalmatia County. Results: All samples must have a detected IPC (Internal Control), which proves that the sample processing procedure was done well, and the interpretation of test results can be accessed. A positive test for SARS-CoV-2 virus indicates the presence of three detectable genes: E, RdRp and N, while for a negative result the genes are not expressed. Conclusion: The fastest, most sensitive and most cost-effective diagnostic method for the detection and quantification of newly emerging SARS-CoV-2 virus is RT-PCR.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:176:700298
Study programmeTitle: Medical Laboratory Diagnostics (university/undergraduate)
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni prvostupnik/ prvostupnica (baccalaureus/ baccalaurea) medicinske laboratorijske dijagnostike (sveučilišni prvostupnik/ prvostupnica (baccalaureus/ baccalaurea) medicinske laboratorijske dijagnostike)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2022-07-21 12:00:07