Title Usporedivost metoda za određivanje kortizola u uzorku 24h mokraće Title (english) Comparability of methods for determining cortisol in a 24-hour urine sample Author Magdalena Baras Mentor Daniela Šupe-Domić (mentor)Committee member Esma Čečuk-Jeličić (predsjednik povjerenstva)Committee member Vanja Kaliterna (član povjerenstva)Committee member Daniela Šupe-Domić (član povjerenstva)Granter University of Split Defense date and country 2024-06-28, Croatia Scientific / art field, BIOMEDICINE AND HEALTHCARE Abstract Nadbubrežna žlijezda sastoji se od srži koja sintetizira kateholamine i kore koja sintetizira steroidne
hormone, pri čemu obje komponente imaju specifične funkcije i regulacije unutar endokrinog sustava.
Hiperfunkcija nadbubrežne žlijezde uzrokovana je prekomjernim lučenjem određenog hormona, što može
dovesti do stanja kao što su hiperaldosteronizam, koji uzrokuje hipertenziju i hipokalijemiju zbog viška
aldosterona ili Cushingov sindrom koji je posljedica dugotrajno povišene razine
... More kortizola, a očituje se
simptomima poput debljanja, slabosti mišića i visokog krvnog tlaka. Hipofunkcija nadbubrežne žlijezde
može biti uzrokovana primarnom, sekundarnom ili tercijarnom insuficijencijom, s mogućim ozbiljnim
komplikacijama poput adrenalne krize, dok hipoaldosteronizam uzrokuje nedostatak aldosterona, a
Addisonovu bolest, često autoimunu, karakterizira postupni početak. Kortizol, hormon stresa koji se
sintetizira iz kolesterola u nadbubrežnim žlijezdama, izlučuje se cirkadijalnim ritmom, regulira se kroz
negativnu povratnu spregu, ključan je za energetski metabolizam, ravnotežu elektrolita te utječe na
imunološki sustav, a njegov metabolizam odvija se kroz pretvaranje u kortizon i daljnji metabolizam.
Analizirano je 40 uzoraka 24h mokraće pacijenata u dobi 25 - 83 godine (30 žena, 10 muškaraca) uz pomoć
dvije metode mjerenja koncentracije kortizola elektrokemiluminiscentnim imunotestom (ECLIA) na Cobas
uređajima proizvođača Roche Diagnostics, uz statističku obradu podataka pomoću "MedCalc "softvera.
Istraživanje je analiziralo distribuciju podataka i usporedilo dvije metode korištenjem KolmogorovSmirnov testa i Passing-Bablokove regresije. Prva metoda, koja uključuje ekstrakciju uzorka, nema
značajno odstupanje od normalne distribucije, dok je druga metoda, bez ekstrakcije, pokazala značajno
odstupanje od normalne distribucije. Rezultati regresije pokazuju značajnu proporcionalnu razliku između
metoda, pri čemu metoda koja uključuje ekstrakciju uzorka daje dosljedno više vrijednosti. Međutim,
sustavna razlika između metoda nije značajna, a visoka korelacija sugerira dosljednost metoda unatoč
proporcionalnim razlikama. Zaključak istraživanja ističe značajne razlike između dviju metoda za mjerenje
kortizola u mokraći. Unatoč proporcionalnim razlikama, visoka korelacija između metoda ukazuje na
konzistentnost mjerenja. Ovi nalazi naglašavaju potrebu za pažljivim razumijevanjem specifičnosti i
kalibracija metoda pri interpretaciji rezultata Less Abstract (english) The adrenal gland consists of a medulla that produces catecholamines and a cortex that produces steroid
hormones, both components having specific functions and regulations within the endocrine system.
Hyperfunction of the adrenal gland is caused by the excessive secretion of a certain hormone, which can
lead to conditions such as hyperaldosteronism, which causes hypertension and hypokalemia due to excess
aldosterone, or Cushing's syndrome, which is the result of long-term elevated
... More cortisol levels, and is
manifested by symptoms such as weight gain, muscle weakness and high blood pressure. Adrenal
hypofunction can be caused by primary, secondary or tertiary insufficiency, with possible serious
complications such as adrenal crisis, while hypoaldosteronism causes a lack of aldosterone, and Addison's
disease, often autoimmune, is characterized by a gradual onset. Cortisol, a stress hormone that is
synthesized from cholesterol in the adrenal glands, secreted in a circadian rhythm, regulated through a
negative feedback loop is crucial for energy metabolism, electrolyte balance and affects the immune system,
and its metabolism takes place through conversion into cortisone and further metabolism. 40 samples of
24h urine from patients aged 25 - 83 (30 women, 10 men) were analyzed with two methods of measuring
cortisol concentration by electrochemiluminescence immunoassay (ECLIA) on Cobas devices
manufactured by Roche Diagnostics, with statistical data processing using "MedCalc" software. The
research analyzed the data distribution and compared the two methods using the Kolmogorov-Smirnov test
and the Passing-Bablok regression. The first method, which includes sample extraction, has no significant
deviation from the normal distribution, while the second method, without extraction, showed a significant
deviation from the normal distribution. The regression results show a significant proportional difference
between the methods, with the method involving sample extraction giving consistently higher values.
However, the systematic difference between the methods is not significant and the high correlation suggests
the consistency of the methods despite the proportional differences. The conclusion of the research
highlights significant differences between the two methods for measuring cortisol in urine. Despite the
proportional differences, the high correlation between the methods indicates the consistency of the
measurements. These findings emphasize the need for careful understanding of method specificity and
calibration when interpreting results. Less Keywords
kortizol
24h mokraća
ECLIA
Keywords (english)
cortisol
24h urine
ECLIA
Language croatian URN:NBN urn:nbn:hr:176:366695 Study programme Title: Undergraduate University Study Programme of Medical Laboratory Diagnostics Type of resource Text File origin Born digital Access conditions Open access Terms of use Created on 2024-06-28 09:49:26
