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undergraduate thesis
Analiza uzoraka ekshumiranih iz masovnih grobnica: od uzorka do identifikacije
2016. urn:nbn:hr:176:037980
Jančić, Ana
University of Split
University Department of Health Studies

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Jančić, A. (2016). Analiza uzoraka ekshumiranih iz masovnih grobnica: od uzorka do identifikacije (Undergraduate thesis). Retrieved from https://urn.nsk.hr/urn:nbn:hr:176:037980

Jančić, Ana. "Analiza uzoraka ekshumiranih iz masovnih grobnica: od uzorka do identifikacije." Undergraduate thesis, University of Split, University Department of Health Studies, 2016. https://urn.nsk.hr/urn:nbn:hr:176:037980

Jančić, Ana. "Analiza uzoraka ekshumiranih iz masovnih grobnica: od uzorka do identifikacije." Undergraduate thesis, University of Split, University Department of Health Studies, 2016. https://urn.nsk.hr/urn:nbn:hr:176:037980

Jančić, A. (2016). 'Analiza uzoraka ekshumiranih iz masovnih grobnica: od uzorka do identifikacije', Undergraduate thesis, University of Split, University Department of Health Studies, accessed 16 March 2023, https://urn.nsk.hr/urn:nbn:hr:176:037980

Jančić A. Analiza uzoraka ekshumiranih iz masovnih grobnica: od uzorka do identifikacije [Undergraduate thesis]. Split: University of Split, University Department of Health Studies; 2016 [cited 2023 March 16] Available at: https://urn.nsk.hr/urn:nbn:hr:176:037980

A. Jančić, "Analiza uzoraka ekshumiranih iz masovnih grobnica: od uzorka do identifikacije", Undergraduate thesis, University of Split, University Department of Health Studies, Split, 2016. Available at: https://urn.nsk.hr/urn:nbn:hr:176:037980

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Metadata
TitleAnaliza uzoraka ekshumiranih iz masovnih grobnica: od uzorka do identifikacije
Title (english)ANALYSIS OF SAMPLES EXHUMED FROM MASS GRAVES: FROM SAMPLE TO IDENTIFICATION
AuthorAna Jančić
MentorDavorka Sutlović (mentor)
Committee memberDaniela Šupe-Domić (predsjednik povjerenstva)
Committee memberMerica Glavina Durdov (član povjerenstva)
Committee memberDavorka Sutlović (član povjerenstva)
GranterUniversity of Split
University Department of Health Studies
Split
Defense date and country2016-07-07, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Clinical Medical Sciences
Abstract
Cilj: Opisati proces identifikacije ljudskih ostataka pronađenih u masovnim grobnicama analizom DNA iz koštanih uzoraka, istaknuti probleme izolacije DNA i nemogućnosti prikupljanja uzoraka DNA pretpostavljene uže rodbine. Metode: Nakon uzimanja uzorka koštanih ostataka, izvršena je DNA ekstrakcija klasičnom organskom ekstrakcijom. Zatim se poslije kontrole dobivene DNA, uporabom lančane reakcije polimerazom (PCR) umnože ciljni fragmenti DNA. Umnoženi se fragmenti vizualiziraju na ABI Prism 310 Genetic Analyzeru. Rezultati: Analize 15 STR lokusa i gena za amelogenin utvrđeni su genotipovi uzorka kosti žrtve i uzoraka krvi pretpostavljene rodbine. Usporedbom podudarnih lokusa utvrđeno je da je 99,986672% vjerojatnost da je xy sin majke xx. Zaključak: Zbog dostupnosti uzoraka pretpostavljene rodbine i postojanja dosta zaživotnih podataka o pretpostavljenim žrtvama Domovinskog rata, analiza koštanih uzoraka rađena je putem analize autosomnih STR-sustava. Identifikacija je izvršena usporedbom genotipa svakog kosturnog ostatka sa genotipovima u postojećoj bazi podataka. Ključne riječi: DNA analiza, identifikacija, uzorci koštanih ostataka
Abstract (english)
Aim: Descirbe the identification of human remains found in mass graves with DNA analysis of skeletal samples, underlain problems with DNA isolation and impossibility of gathering DNA samples from assumed close relatives. Methods: After taking bone samples, DNA extraction is done by classical organic extraction method. Afterwards DNA control is done and by use of polymerase chain reaction (PCR) target fragments of DNA are amplified. Multiplied fragments are visualized on ABI Prism 310 Genetic Analyzer. Results: With analysis of 15 STR loci and amelogenin gen, genotypes from victims bone samples and samples from assumed close relatives are established. Comparation of matching loci stated it is 99, 986672% probability that xy is son of mother xx. Conclusion: Due to availabilty samples assumed close relatives and existence of pre-mortem data about assumed victims in 1991-1995 war, bone samples analysis is executed by autosomal STR- systems. Identification is done by comparation of genotype of every skeletal remain with genotypes in existing data base. Key words: DNA analysis, identification, samples of skeletal remains, bone samples
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:176:037980
Study programmeTitle: Medical Laboratory Diagnostics (university/undergraduate)
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni prvostupnik/prvostupnica (baccalaureus/baccalaurea) medicinska laboratorijska dijagnostika (sveučilišni prvostupnik/prvostupnica (baccalaureus/baccalaurea) medicinska laboratorijska dijagnostika)
Type of resourceText
File originBorn digital
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Created on2020-06-01 10:53:12