Forensic radiology, as a specialist branch of radiology, provides answers to medical and legal aspects with an emphasis on medical expertise. Their purpose is to conduct legal proceedings, and includes reports based on radiological examinations and procedures. Various radiological methods are often used to determine the cause of death, age estimation and identification. Countless radiological techniques are used to aid in human identification. Assessing the age of living persons or skeletal remains is a common requirement in forensic practice and bioarchaeology. For living persons, age estimation can be conducted under a number of circumstances such as determing criminal responsibility, seeking asylum, school attendance, employment and marriage while in forensic anthropology and forensic odontology age estimationis an important step in identifying human remains. Indicators of physiological maturity are dental, skeletal physical and mental maturity, which we compare with the chronological age of the patient. The chronological age of the patient is the time elapsed since the birth of the individual. Some experts consider (presume) that age estimation in old age (50+) is all but impossible (practically) because deviations in the results and thus inaccuracy of the method are so great that any attempt to narrow the interval is in vain. Bones are the calcified connective tissue that makes up the main part of the skeleton of most vertebrates. From the moment of birth to the moment when one grows up, the bones go through a set of characteristic changes. Therefore, bone maturity or bone age can be assessed on radiographic images of specific bones in the human body. Osteological determination of age is very challenging today due to the many complex issues and problems it opens up. Bone changes which aretakenintoaccount do not depend exclusively on age but on the interaction of the number of external and internal factors (genes, cultural and social factors, environmental factors). In clinical practice, the most commonly used is comparison of x-rays of the wrist of the hand with standard images in the corresponding atlases (the most famous Greulich – Pyle atlas). Another way to estimate bone age is according to the Tanner and Whitehouse method so-called TW2 method. Teeth (lat. dens) are solid mineralized olfactory organs located in the bones of the upper and lower jaw, which belong to the oral cavity. Teeth are not as exposed to socioeconomic impact as bones. Over time, dental radiology has developedthat plays an important role in age estimation. It provides insight into the mineralization of the crown and root of the tooth. The best insight into the development and mineralization of all teeth can be achieved using orthopanthomographic images. Although there are several approaches to age assessment but still there is no universal procedure and formula that would be applied with the same success to members of all nations and groups. Nevertheless, any research that will evaluate possible inclusions and combinations of all useful anatomical regions in determing the age limit in age estimation or answering a particular question e.g. whether a person is an adult or a minor is welcome. Also, recent research has made a significant step forward in the application of non-invasive imaging procedures in assessing the age of living subjects such as magnetic resonance imaging (MR) and ultrasound examinations.