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undergraduate thesis
Primjena imunofluorescencijske tehnike u analizi karcinoma bubrega čovjeka
2020. urn:nbn:hr:176:961207
Franjkić, Toni
University of Split
University Department of Health Studies

Cite this document

Franjkić, T. (2020). Primjena imunofluorescencijske tehnike u analizi karcinoma bubrega čovjeka (Undergraduate thesis). Split: University of Split. Retrieved from https://urn.nsk.hr/urn:nbn:hr:176:961207

Franjkić, Toni. "Primjena imunofluorescencijske tehnike u analizi karcinoma bubrega čovjeka." Undergraduate thesis, University of Split, 2020. https://urn.nsk.hr/urn:nbn:hr:176:961207

Franjkić, Toni. "Primjena imunofluorescencijske tehnike u analizi karcinoma bubrega čovjeka." Undergraduate thesis, University of Split, 2020. https://urn.nsk.hr/urn:nbn:hr:176:961207

Franjkić, T. (2020). 'Primjena imunofluorescencijske tehnike u analizi karcinoma bubrega čovjeka', Undergraduate thesis, University of Split, accessed 12 December 2024, https://urn.nsk.hr/urn:nbn:hr:176:961207

Franjkić T. Primjena imunofluorescencijske tehnike u analizi karcinoma bubrega čovjeka [Undergraduate thesis]. Split: University of Split; 2020 [cited 2024 December 12] Available at: https://urn.nsk.hr/urn:nbn:hr:176:961207

T. Franjkić, "Primjena imunofluorescencijske tehnike u analizi karcinoma bubrega čovjeka", Undergraduate thesis, University of Split, Split, 2020. Available at: https://urn.nsk.hr/urn:nbn:hr:176:961207

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Metadata
TitlePrimjena imunofluorescencijske tehnike u analizi karcinoma bubrega čovjeka
Title (english)APPLICATION OF IMMUNOFLUORESCENT TECHNIQUE IN HUMAN KIDNEY CARCINOMA ANALYSIS
AuthorToni Franjkić
MentorKatarina Vukojević (mentor)
Committee memberSnježana Mardešić (predsjednik povjerenstva)
Committee memberEsma Čečuk-Jeličić (član povjerenstva)
Committee memberKatarina Vukojević (član povjerenstva)
GranterUniversity of Split
(University Department of Health Studies)
Split
Defense date and country2020-07-21, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Clinical Medical Sciences
Abstract
Karcinom bubrega maligna je bolest koja spada među najčešće karcinome unutar svjetske populacije te predstavlja šesnaesti najčešći uzrok smrti unutar svjetske populacije. Rizične skupine za oboljevanje od karcinoma bubrega jesu pušači, osobe koje pate od hipertenzije, osobe koje konzumiraju hranu koja sadrži velike količine proteina i masti te osobe koje konzumiraju velike količine povrća i antioksidansa. Cilj ovog istraživanja bio je objasniti patohistološke metode dijagnostike karcinoma bubrega analizom histopatoloških preparata te primjenom imunofluorescencijske tehnike u analizi karcinoma i objasniti važnost imunofluorescencijske tehnike u točnosti i specifičnosti postavljanja dijagnoze za navedenu bolest. Primjenom imunofluorescencijskih tehnika u analizi navedene bolesti, značajno je olakšana dijagnostika i praćenje stupnja napredovanja tkivnog karcinoma. Osnovu imunofluorescencijskih tehnika predstavlja reakcija između antigena i antitijela te stvaranje kompleksa antigen-antitijelo. Proces analize tkivnih preparata obuhvaća postupke zaprimanja uzroka, pripreme i uklapanja tkiva u parafinske blokove te deparafinizaciju tkiva i aplikaciju primarnih i sekundarnih antitijela koji mogu pomoći u dijagnostici karcinoma bubrega, budući da ekspresija istih može ukazati na faze napredovanja bubrežnog karcinoma. Analiza predmetnih stakalaca radi se uz pomoć fluorescencijskog mikroskopa te, u konačnici, na računalnim programima koji omogućuju brojanje stanica koje pokazuju ekspresiju, tj. pozitivne signale u malignoj bolesti bubrežnog tkiva. Istraživanje je obuhvatilo analizu dvanaest predmetnih stakalaca tkiva zahvaćenih karcinomom, od čega je na četiri tkiva analiza bila otežana zbog prisustva interferencija (artefakata). Analizom PTEN markera sugerira se kako pokazuje slabu ekspresiju na stanicama bubrežnog tkiva zahvaćenog karcinomom te isti biva inhibiran što je karcinom u uznapredovaloj fazi razvoja. SATB1 pokazuje izrazitu ekspresiju na stanicama bubrežnog tkiva zahvaćenog karcinomom. SATB1 marker pokazuje pozitivnu ekspresiju unutar različitih oblika bubrežnih maligniteta. Primjena normiranih smjernica propisanih za pravilnu analizu karcinoma bubrega te poštivanje pravila dobre laboratorijske prakse, nužna su za kvalitetni postupak izrade i analize tkivnog preparata za postavljanje dijagnoze bubrežne bolesti. Buduća istraživanja potrebno je usmjeriti razvoju preciznih i visoko sofisticiranih tehnika za mogućnost optimalne analize tkivnih preparata zahvaćenih karcinomima.
Abstract (english)
Kidney carcinoma is malignant disease which is one of the most common carcinomas in the global population and it is sixteenth cause of the mortality in the total world population. Risk groups for morbidity of mentioned disease are smokers, hypertensive patients, persons who consume food which contains lot of proteins and fat and persons who consume lot of vegetables and antioxidants. The aim of this study was to explain patohistological diagnostic methods of human kidney carcinoma analysis using histopathological techniques, apply immunofluorescence techniques in human kidney carcinoma analysis and explain the importance of immunofluorescent technique in accuracy and specificity of diagnosis for discussed disease. By using immunofluorescent techniques in analysis of kidney carcinoma, diagnosis became easier as well as disease monitoring through longer period. The basic of immunofluorescent techniques presents reaction between antigen and antibody and creation of antigen-antibody complex. Process of analyzing tissue samples includes procedure of receiving sample, preparation and incorporation of tissue in paraffin blocks, deparaffinisation and application of primary and secondary antibodies which present phase of human kidney carcinoma progession. Analysis of tissue samples is being worked on the fluorescence microscope and in computer programs which enable cell counting which show expression, with positive signals in malignant disease of kidney tissue. Research involve analysis of twelve tissue samples with kidney carcinoma, of which four were demanding because of artifacts (interference). Analysis of PTEN marker suggests low expression on human kidney carcinoma cells and the same marker is inhibited by progression of human kidney carcinoma. SATB1 shows high level of expression on human kidney carcinoma cells. SATB1 suggests positive expression between different type of kidney malignancy. Use of standardized guidelines provided for human kidney carcinoma analysis and working in compliance of good laboratory practice, are necessary for high quality of tissue analysis for diagnosis of malignant kidney disease. Future researches need to be directed in development of precise and high sophisticated techniques for optimal malignant tissue analysis.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:176:961207
Study programmeTitle: Medical Laboratory Diagnostics (university/undergraduate)
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni prvostupnik/prvostupnica (baccalaureus/baccalaurea) medicinsko laboratorijske dijagnostike (sveučilišni prvostupnik/prvostupnica (baccalaureus/baccalaurea) medicinsko laboratorijske dijagnostike)
Type of resourceText
File originBorn digital
Access conditionsAccess restricted to students and staff of home institution
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Created on2021-02-03 10:51:45