Hepatitis B is contagious liver disease caused by HBV. There are several types of hepatitis, but hepatitis B is the most widespread type of hepatitis. It is reported in different ways. In developed countries and in Croatia predominant route of transmission is through sexual contact, while in poor countries the dominant transmission is by the blood of an infected person. The main goal of treatment is to prevent viral replication. The most effective measure in the fight against HBV is prevention. The aim of this paper is to specify and describe forms of prevention (primary, secondary, tertiary) and the role of nurses in the prevention of hepatitis B. Primary prevention refers to the healthy population. The role of nurse includes the enforcement of compulsory vaccination and education, and health education of all ages. Secondary prevention refers to the "potential" patients. The role of nurse is to educate them on how to transfer the virus and avoiding risky behavior. Transfer the blood of an infected HBV is the most common route of transmission among health care workers. Because medical staff carried out general and specific measures to prevent the occurrence and spread of the infection. Tertiary prevention refers to patients in whom treatment fails to prevent the development of disease. Nurses approach to patients requires knowledge and skill, and experience, in order to assist patients in an appropriate manner in all states of fear and discomfort that can permeate in their coping with the disease. Key words: hepatitis B, nurse, prevention