The aim of this paper is to determine how many women, given their age and level of education, responded to a public health intervention after an invitation for mammography within the National Program for Early Detection of Breast Cancer (“NPP”) in 2018, at the Zagreb County Health Center - Zaprešić Regional Health Center. It was also necessary to examine whether the education level and age were related to the response to the public health invitation for NPP and determine the possibilities of increasing the response to the public health invitation for NPP for early detection of breast cancer. A retrospective study of medical documentation used data regarding the age and level of education of patients who voluntarily responded to the public health call for a diagnostic mammography procedure in the period from January to December 2018 at the Zagreb County Health Center - Zaprešić Regional Health Center. Testing the heterogeneity of the distribution within the patient population according to the level of education based on the sample was performed using the χ2-test. Descriptive statistics methods were used to present mean values and their dispersion indicators. Statistical calculators were used in the calculation and comparison of the obtained results with external sources, namely, the Z-test difference calculator in proportions in two independent sets, and the T-test calculator. The analysis was performed in the statistical program SPSS 25. The determined mean age of women who responded to the invitation for mammography within the National Program for Early Detection of Breast Cancer (Zaprešić Regional Health Center, 2018) was 61 (IQR = 56.00-66.00), which shows that the youngest age group does not provide a higher response to mammography within the NPP. Women with a lower degree of education (unskilled workers) are less likely to respond to the invitation for mammography within the National Program for Early Detection of Breast Cancer (Zaprešić Regional Health Center, 2018), and the study did not find a difference in response between women with secondary, undergraduate and graduate education level, with regard to their representation in the educational structure of women in the general population (Census of the Republic of Croatia, 2011). It can be concluded that the activities within prevention measures, more specifically, the National Program for Early Detection of Breast Cancer, should be adapted and directed towards the level of education of all women, and in order to achieve this, it is necessary to include a multidisciplinary team of public health experts, radiologists and other health professionals.