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undergraduate thesis
Uloga PCR tehnologije u detekciji Covid-19 infekcije
2021. urn:nbn:hr:176:353178
Turkalj, Iva
University of Split
University Department of Health Studies

Cite this document

Turkalj, I. (2021). Uloga PCR tehnologije u detekciji Covid-19 infekcije (Undergraduate thesis). Split: University of Split. Retrieved from https://urn.nsk.hr/urn:nbn:hr:176:353178

Turkalj, Iva. "Uloga PCR tehnologije u detekciji Covid-19 infekcije." Undergraduate thesis, University of Split, 2021. https://urn.nsk.hr/urn:nbn:hr:176:353178

Turkalj, Iva. "Uloga PCR tehnologije u detekciji Covid-19 infekcije." Undergraduate thesis, University of Split, 2021. https://urn.nsk.hr/urn:nbn:hr:176:353178

Turkalj, I. (2021). 'Uloga PCR tehnologije u detekciji Covid-19 infekcije', Undergraduate thesis, University of Split, accessed 17 November 2024, https://urn.nsk.hr/urn:nbn:hr:176:353178

Turkalj I. Uloga PCR tehnologije u detekciji Covid-19 infekcije [Undergraduate thesis]. Split: University of Split; 2021 [cited 2024 November 17] Available at: https://urn.nsk.hr/urn:nbn:hr:176:353178

I. Turkalj, "Uloga PCR tehnologije u detekciji Covid-19 infekcije", Undergraduate thesis, University of Split, Split, 2021. Available at: https://urn.nsk.hr/urn:nbn:hr:176:353178

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Metadata
TitleUloga PCR tehnologije u detekciji Covid-19 infekcije
Title (english)The role of PCR tehchnology in COVID-19 detection
AuthorIva Turkalj
MentorDavorka Sutlović (mentor)
MentorSendi Kuret (sumentor)
Committee memberEsma Čečuk-Jeličić (predsjednik povjerenstva)
Committee memberAntonela Matana (član povjerenstva)
Committee memberDavorka Sutlović (član povjerenstva)
GranterUniversity of Split
(University Department of Health Studies)
Split
Defense date and country2021-07-01, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Clinical Medical Sciences
Abstract
Cilj: Istražiti ulogu PCR tehnologije pri dijagnostici COVID-19 infekcije. Materijal i metode:Opisan je postupak izvođenja PCR-a od izolacije RNA do detekcije i amplifikacije virusa na dva aparata kao i postupak i svrha brzog PCR testiranja. Također, opisani su prikladni materijali za postupak RT-PCR-a i brzog PCR testa. Rezultati:PCR produkt nastaje eksponencijalno. Potrebno je nekoliko ciklusa da bi se umnožilo dovoljno DNA kopija, krivulja odnosa intenziteta fluorescencije prema broju ciklusa pokazuje sigmoidalni oblik. U kasnim ciklusima supstrati reakcije su potrošeni i PCR produkt se više ne udvostručuje, krivulja prelazi u ravan oblik. Važna je Ct vrijednost koja je obrnuto proporcionalna količini virusa prisutnoj u uzorku. Zaključak: Obje metode, RT-PCR i brzi PCR test, imaju visoku osjetljivost i specifičnost kod pozitivnih rezultata no RT-PCR ima manju stopu lažno negativnih rezultata.
Abstract (english)
Objectives: To investigate the role of PCR technology in the diagnosis of COVID-19 infection. Material and methods: The procedure for performing PCR from RNA isolation to virus detection and amplification is described as well as the procedure and purpose of rapid PCR testing. Suitable materials for RT- PCR and rapid PCR testing are also described. Results: PCR product is formed exponentially. It takes several cycles to replicate enough DNA copies, the flourescence intensity-to number ratio curve shows the sigmoidal shape. In the late cycles the reaction substrates are consumed and the PCR product no longer doubles, the curve turns into a flat shape. Ct value, which is inversely proportional to the amount of virus present in the sample, is also important. Conclusion: Both methods have high sensitivity and specificity in positive results but RT-PCR has lower rate of false negative results.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:176:353178
Study programmeTitle: Medical Laboratory Diagnostics (university/undergraduate)
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni prvostupnik/ prvostupnica (baccalaureus/ baccalaurea) medicinske laboratorijske dijagnostike (sveučilišni prvostupnik/ prvostupnica (baccalaureus/ baccalaurea) medicinske laboratorijske dijagnostike)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2021-07-01 18:34:16