Aim of the research: The aim of this study was to examine the knowledge and attitudes of nurses about transfusion therapy. To determine whether there is a statistically significant difference in knowledge about transfusion treatment between nurses of secondary education and above. To determine whether there is a statistically significant difference in knowledge between nurses with regard to their work experience, ie work experience in the profession. Methods: The research was conducted in the period from 18.05.2021 to 08.06.2021. using a questionnaire compiled with the help of a mentor Doc.dr.sc. Dejane Bogdanić dr.med. The survey consists of 9 questions of different types that the respondents filled in independently. The paper uses methods of graphical and tabular presentation which present the structure of answers to the survey questions. Numerical values are presented using the methods of descriptive statistics, namely arithmetic means and standard deviations. In case of deviation from the normal distribution, the median and interquartile range are used. The normality of the distribution was previously checked by the Kolmogorov-Smirnov test. Knowledge is presented using a percentage point scale with a range of values from 0 to 100% where respondents who did not know the answer to any question get 0, and respondents who knew all the answers get 100%. The range of correct answers is presented using both the minimum and maximum values. The Hi square test and the Kruskal-Wallis test are used to test the hypotheses. The analysis was performed in the statistical software STATISTICA 12 (TIBCO Software Inc., Palo Alto, California). Research results: The research established that the largest number of respondents have completed secondary education, and that the average age of respondents is 39 years. Thus, we can conclude that the respondents are younger. The average length of work experience is 17 years. The largest number of respondents is employed at the Clinic for Internal Medicine, and according to the number of blood and blood derivatives that are used in a week at the worke place is 2-5 doses. Research has also shown that nurses prepare the patient, blood derivative and transfusion system, which in 83% of cases they apply themselves. In 66% of cases, the doctor comes only by call. A large number of respondents believe that it is necessary to conduct additional education of employees on the use of blood and blood derivatives. A large number of respondents agree that donating blood does not harm health and does not weaken the immune system. A smaller percentage of respondents (40%) believe that transfusion treatment is safe and effective, and 25% of respondents are aware that the use of blood derivatives can infect a patient with hepatitis or some other transfusion transmissible disease. Respondents have positive attitudes about transfusion treatment, and expressed a high level of knowledge on all survey questions where the mean value of correct answers is 80%. Respondents from the Oncology Clinic have the highest level of knowledge (85%), and employees of the Clinic for Women's Diseases and Obstetrics have the lowest level of knowledge (57%), which suggests that employees who are more likely to have experience in applying blood transfusion have a higher level of knowledge. Conclusion: In our research, we showed that the respondents (nurses of KBC Split) have a high level of knowledge about transfusion treatment. Also, we determined that additional education is needed at the Clinic for Women's Diseases and Obstetrics, and additional education of doctors on the need to monitor patients transfusion treatment. In the research, we proved that the level of knowledge depends on education, but not on years of work experience.