INTRODUCTION: Blood transfusion is a procedure that started being used more frequently in 20th century as a way of treating patients thanks to the advances in the fields of microbiology and other branches of medicine. The main source of blood and blood products are voluntary blood donors. With the goal of preventing spread of blood-borne diseases, every dose of donated blood is tested with serological and NAT methods, and to prevent hemolytic transfusion reactions the same blood is tested with imunohematological methods. Among other tests, direct antiglobulin tests (DAT) and indirect antiglobulin (IAT) tests are part of imunohematological testing. OBJECTIVE: To determine the frequency of positive direct antiglobulin tests and indirect antiglobulin tests and to determine the specificity of irregular antibodies in voluntary blood donors in the observed period of time from January 1st, 2019 to December 31st, 2020. METHODS: The methods used to examine the results of DATS are classical test tube method and the microhemagglutination method. In case of a positive result, the blood products are withdrawn from use and the donors return to the control DAT one month after the positive result. Depending on the result of the latter, donors are either prohibited from donating blood for 2 years or are allowed to donate. The method by which the IAT is tested is called the microhemagglutination method. In the case of a positive IAT, all blood products are withdrawn from use and the donors return to the control IAT one month after the positive result. Depending on the result of the control IAT, donors are either prohibited from donating blood for 5 years or are allowed to continue donating blood. RESULTS: In the two-year observed period, there were 19 blood products with a positive DAT out of 38 051 collected, thus having a share of 0,5‰. Of the mentioned 19 respondents, after the control testing, 10 of them were prohibited from donating for 2 years, and 9 of them were allowed to donate further. There were 20 positive IATs in the reference period, or 0,5‰ of the total collected. The most common irregular antibody that caused 8 or 40% of positive IATs was anti-M antibody. The least frequent antibodies are anti-c and anti-Jka antibodies, each of which has a share of 10% or 2 positive findings. Anti-c antibodies caused both positive IATs in 2019 and anti-Jka antibodies caused both positive IATs in 2020. CONCLUSION: Positive DATs were rare in the reference period, recording their share of 0.05% or 19 of the total collected doses of whole blood. A similar percentage was recorded for IATs with 20 or 0.05% positive results against the total number of whole blood doses collected. The most common irregular antibody that causes positive IATs is anti-M antibody, and the least common are anti-c and anti-Jka antibodies.