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undergraduate thesis
Skraćena validacija HPLC metode za određivanje HbA1c
2017. urn:nbn:hr:176:963607
Lučin, Dora
University of Split
University Department of Health Studies

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Lučin, D. (2017). Skraćena validacija HPLC metode za određivanje HbA1c (Undergraduate thesis). Split: University of Split, University Department of Health Studies. Retrieved from https://urn.nsk.hr/urn:nbn:hr:176:963607

Lučin, Dora. "Skraćena validacija HPLC metode za određivanje HbA1c." Undergraduate thesis, University of Split, University Department of Health Studies, 2017. https://urn.nsk.hr/urn:nbn:hr:176:963607

Lučin, Dora. "Skraćena validacija HPLC metode za određivanje HbA1c." Undergraduate thesis, University of Split, University Department of Health Studies, 2017. https://urn.nsk.hr/urn:nbn:hr:176:963607

Lučin, D. (2017). 'Skraćena validacija HPLC metode za određivanje HbA1c', Undergraduate thesis, University of Split, University Department of Health Studies, accessed 04 June 2023, https://urn.nsk.hr/urn:nbn:hr:176:963607

Lučin D. Skraćena validacija HPLC metode za određivanje HbA1c [Undergraduate thesis]. Split: University of Split, University Department of Health Studies; 2017 [cited 2023 June 04] Available at: https://urn.nsk.hr/urn:nbn:hr:176:963607

D. Lučin, "Skraćena validacija HPLC metode za određivanje HbA1c", Undergraduate thesis, University of Split, University Department of Health Studies, Split, 2017. Available at: https://urn.nsk.hr/urn:nbn:hr:176:963607

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Metadata
TitleSkraćena validacija HPLC metode za određivanje HbA1c
Title (english)SHORT VALIDATION OF HPLC METHOD FOR DETERMINATION HBA1C
AuthorDora Lučin
MentorLeida Tandara (mentor)
Committee memberDaniela Šupe-Domić (predsjednik povjerenstva)
Committee memberFrane Mihanović (član povjerenstva)
Committee memberLeida Tandara (član povjerenstva)
GranterUniversity of Split
University Department of Health Studies
Split
Defense date and country2017-07-20, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Clinical Medical Sciences
Abstract
UVOD: Eritrociti sadrže 95% do 98% adultnog hemoglobina (HbA), a ostalo su produkti alternativne sinteze globina i post-translacijskih modifikacija HbA. Hemoglobin A1c (HbA1c) najbrojnija je frakcija HbA. HbA1c danas se koristi kao standardna mjera prosječne glikemije te služi za rutinsko praćenje i prevenciju dugoročnih komplikacija šećerne bolesti jer odražava prosječnu koncentraciju glukoze u krvi tijekom prethodna 2 do 3 mjeseca. Današnji klinički normativ za procjenu učinkovitosti terapije i rizika pojave komplikacija šećerne bolesti čini dobra kontrola glikemije, izražena kroz vrijednost HbA1c ≤ 7%. MATERIJALI I METODE: Usporedba metoda: imunokemijske i HPLC metode provedena je korištenjem rutinskih uzoraka pacijenata koji su upućeni u laboratorij radi standardne dijagnostičke obrade. Ispitano je 40 uzoraka pune krvi pacijenata na analizatorima Roche Cobas Integra 400 plus te Humanex A1c. CILJEVI: Cilj ovog istraživanja bio je provesti analitičku evaluaciju usporedivosti rezultata za HbA1c dobivenih različitim metodama. REZULTATI: Rezultati dobiveni Passing Bablokovom i Demingovom regresijskom analizom pokazuju da su metode u potpunosti usporedive jer nema ni konstantnog ni proporcionalnog odstupanja između rezultata dobivenih navedenim metodama. HPLC metoda koja se uvodila u rutinski rad pokazala je zadovoljavajuću preciznost. ZAKLJUČAK: Usporednim određivanjem pokazalo se da ne postoje značajna odstupanja u rezultatima dobivenim ispitivanim metodama što je iznimno značajno zbog potrebe longitudinalnog praćenja bolesnika sa šećernom bolešću. KLJUČNE RIJEČI: HbA1c, šećerna bolest, usporedba metoda
Abstract (english)
INTRODUCTION: Erythrocytes contain about 95% to 98% of adult hemoglobin (HbA), and small amount of other products of alternative globin synthesis and post-translational modification of HbA. Hemoglobin A1c (HbA1c) is the largest fraction of HbA. HbA1c is now used for routine monitoring and prevention of long-term diabetes complications because it reflects the average blood glucose concentration over the previous 2 to 3 months. Today's clinical standard for assessing the efficacy of the therapy and the risk of complications of diabetes is good glycemic control, expressed through HbA1c ≤ 7%. MATERIALS AND METHODS: 40 full-blood samples were analyzed on Roche Cobas Integra 400 plus and Humanex A1c analyzers using routine patient samples taken during standard diagnostic treatment at a hospital. Comparison of methods, immunoassay method and HPLC was performed. OBJECTIVES: The aim of this study was to evaluae comparability of rezults for HbA1c obtained by two different methods. RESULTS: The results obtained by Passing Bablok and Deming's regression analysis show that methods are completely comparable because there is no constant or proportional deviation between the results obtained by the mentioned methods. HPLC method that was introduced into routine work proved the satisfactory precision. CONCLUSION: It was shown that there were no significant differences in the results of the investigated methods, which is extremely important because of the longitudinal monitoring of patients with diabetes mellitus. KEY WORDS: HbA1c, diabetes, comparison of the methods
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:176:963607
Study programmeTitle: Medical Laboratory Diagnostics (university/undergraduate)
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni prvostupnik/prvostupnica (baccalaureus/baccalaurea) medicinska laboratorijska dijagnostika (sveučilišni prvostupnik/prvostupnica (baccalaureus/baccalaurea) medicinska laboratorijska dijagnostika)
Type of resourceText
File originBorn digital
Access conditionsOpen access
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Created on2020-04-28 09:06:06