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undergraduate thesis
Utvrđivanje očinstva/majčinstva primjenom analize DNA
2016. urn:nbn:hr:176:778513
Milardović, Anamarija
University of Split
University Department of Health Studies

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Milardović, A. (2016). Utvrđivanje očinstva/majčinstva primjenom analize DNA (Undergraduate thesis). Split: University of Split. Retrieved from https://urn.nsk.hr/urn:nbn:hr:176:778513

Milardović, Anamarija. "Utvrđivanje očinstva/majčinstva primjenom analize DNA." Undergraduate thesis, University of Split, 2016. https://urn.nsk.hr/urn:nbn:hr:176:778513

Milardović, Anamarija. "Utvrđivanje očinstva/majčinstva primjenom analize DNA." Undergraduate thesis, University of Split, 2016. https://urn.nsk.hr/urn:nbn:hr:176:778513

Milardović, A. (2016). 'Utvrđivanje očinstva/majčinstva primjenom analize DNA', Undergraduate thesis, University of Split, accessed 10 December 2024, https://urn.nsk.hr/urn:nbn:hr:176:778513

Milardović A. Utvrđivanje očinstva/majčinstva primjenom analize DNA [Undergraduate thesis]. Split: University of Split; 2016 [cited 2024 December 10] Available at: https://urn.nsk.hr/urn:nbn:hr:176:778513

A. Milardović, "Utvrđivanje očinstva/majčinstva primjenom analize DNA", Undergraduate thesis, University of Split, Split, 2016. Available at: https://urn.nsk.hr/urn:nbn:hr:176:778513

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Metadata
TitleUtvrđivanje očinstva/majčinstva primjenom analize DNA
Title (english)PATERNITY/ MATERNITY TESTING USING DNA ANALYSIS
AuthorAnamarija Milardović
MentorDavorka Sutlović (mentor)
Committee memberDaniela Šupe-Domić (predsjednik povjerenstva)
Committee memberMerica Glavina Durdov (član povjerenstva)
Committee memberDavorka Sutlović (član povjerenstva)
GranterUniversity of Split
(University Department of Health Studies)
Split
Defense date and country2016-07-14, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Clinical Medical Sciences
Abstract
Ciljevi rada: Osnovni cilj ovog rada je analizom DNA molekule dokazati te izračunati vjerojatnost očinstva iz dobivenih uzoraka osoba koje su vrlo vjerojatno u srodstvu. Metode: Nakon uzimanja uzoraka brisom bukalne sluznice, iz uzoraka je izolirana DNA molekula pomoću metode sa Chelex®, a zatim je ta ista DNA molekula umnožena metodom lančane reakcije polimerazom, odnosno PCR metodom. Umnožena DNA se razdvaja na ulomke pomoću ABI Prism 310 instrumetna, a na kraju se rezultati očitavaju, uspoređuju te se izračunava statistička vjerojatnost. Rezultati: Nizom analiza genskih lokusa pretpostavljenog oca, majke te djeteta, dokazano je da je mogući otac uistinu otac djetetu sa vjerojatnošću 99,99997 %, te se kao takav uključuje kao biološki otac. Zaključci: Nakon pravilne analize DNA te dobivenih rezultata, ukoliko postoji podudarnost između genskih lokusa djeteta i mogućeg oca, govori se o uključenju ispitivane osobe kao oca, a u suprotnom slučaju, ukoliko se rezultati razlikuju u 2 ili više lokusa, radi se o isključenju. Ključne riječi: DNA molekula, geni, PCR, dokazivanje očinstva
Abstract (english)
Objective: The main aim of this work is to prove and calculate the probability of paternity by analyzing the DNA molecule from the samples received of people who are probably related. Methods: After sampling buccal swab, DNA molecule was first isolated by Chelex® method and then amplified by polymerase chain reaction, or PCR method. The amplified DNA is separated into fractions using an ABI Prism 310 instruments, and finally, the results are read, comparing and calculating the statistical probability. Results: Series of analyzes of genetic loci presumed father, mother and child have proved that the potential father truly is the father of a child with a probability of 99.99997%, and as such includes as the biological father . Findings: After the correct DNA analysis and the results, if there is a correspondence between genetic loci of child and possible father, we consider it as the inclusion of test person as a father, and in the opposite case, if the results are different in two or more loci, it's about exclusion. Key words: DNA molecule, genes, PCR, paternity testing
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:176:778513
Study programmeTitle: Medical Laboratory Diagnostics (university/undergraduate)
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni prvostupnik/prvostupnica (baccalaureus/baccalaurea) medicinska laboratorijska dijagnostika (sveučilišni prvostupnik/prvostupnica (baccalaureus/baccalaurea) medicinska laboratorijska dijagnostika)
Type of resourceText
File originBorn digital
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Created on2020-06-01 11:16:10