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undergraduate thesis
Određivanje NK stanica protočnom citometrijom
2020. urn:nbn:hr:176:044037
Erceg, Klara
University of Split
University Department of Health Studies

Cite this document

Erceg, K. (2020). Određivanje NK stanica protočnom citometrijom (Undergraduate thesis). Split: University of Split. Retrieved from https://urn.nsk.hr/urn:nbn:hr:176:044037

Erceg, Klara. "Određivanje NK stanica protočnom citometrijom." Undergraduate thesis, University of Split, 2020. https://urn.nsk.hr/urn:nbn:hr:176:044037

Erceg, Klara. "Određivanje NK stanica protočnom citometrijom." Undergraduate thesis, University of Split, 2020. https://urn.nsk.hr/urn:nbn:hr:176:044037

Erceg, K. (2020). 'Određivanje NK stanica protočnom citometrijom', Undergraduate thesis, University of Split, accessed 05 April 2025, https://urn.nsk.hr/urn:nbn:hr:176:044037

Erceg K. Određivanje NK stanica protočnom citometrijom [Undergraduate thesis]. Split: University of Split; 2020 [cited 2025 April 05] Available at: https://urn.nsk.hr/urn:nbn:hr:176:044037

K. Erceg, "Određivanje NK stanica protočnom citometrijom", Undergraduate thesis, University of Split, Split, 2020. Available at: https://urn.nsk.hr/urn:nbn:hr:176:044037

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Metadata
TitleOdređivanje NK stanica protočnom citometrijom
Title (english)DETERMINATION OF NK CELLS BY FLOW CYTOMETRY
AuthorKlara Erceg
MentorNada Bilopavlović (mentor)
Committee memberMario Marendić (predsjednik povjerenstva)
Committee memberEsma Čečuk-Jeličić (član povjerenstva)
Committee memberNada Bilopavlović (član povjerenstva)
GranterUniversity of Split
(University Department of Health Studies)
Split
Defense date and country2020-07-31, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Clinical Medical Sciences
Abstract
Uvod: Limfociti pripadaju lozi leukocita i čine ih tri subpopulacije, a to su T i B limfociti i NK stanice. T i B limfociti pripadaju stečenoj imunosti dok NK stanice imaju obilježja prirođene imunosti. Morfološki se navedene populacije stanica ne razlikuju. NK stanicama za citolitičku aktivnost protiv stranog antigena nije potrebna podudaranost s MHC molekulama. Leukocitni diferencijacijski antigeni određuju staničnu lozu i stadij zrelosti, a nazivaju se CD antigenima. NK stanice su CD3- i... More CD16+56. Metodom imunofluorescencije CD antigeni se obilježavaju pomoću anti-CD protutijela na koja su vezani fluorokromi. Protočna citometrija je dijagnostička metoda koja omogućuje imunofenotipizaciju stanica na temelju staničnih biljega (CD biljega). Protočnom citometrijom uz primjenu imunofenotipizacije moguće je odrediti NK stanice u perifernoj krvi odnosno udio unutar limfocita i apsolutni broja u krvi. Prikaz rezultata protočne citometrije je najčešće u obliku citograma. Cilj rada: Svrha rada je bila odrediti apsolutni broj NK stanica metodom protočne citometrije iz uzorka periferne krvi. Materijali i metode: Obrađen je uzorak jednog pacijenta zaprimljenog u Zavodu za medicinsko laboratorijsku dijagnostiku KBC-a Split. Nakon analize krvi na hematološkom brojaču slijedila je obrada uzorka bojanjem fluorescentno obilježenim antitijelima na CD45, CD3 i CD16+56, a potom liziranje i ispiranje eritrocita. Obrađeni uzorak analiziran je na protočnom citometru FACS Calibur. Rezultati: Metodom analize podataka dobivenih protočnom citometrijom iz tri odabrana odgovarajuća citograma dobiveni su podatci o udjelu limfocita unutar leukocita i udjelu NK stanica unutar populacije limfocita. Apsolutni broj NK stanica dobiven je matematičkim izračunom korištenjem broja leukocita izmjerenog hematološkim brojačem Sysmex XN-1000. Zaključak: Za određivanje NK stanica potrebno je primijeniti metodu imunofenotipizacije. U ispitivanom uzorku određen je normalan udio i broj NK stanica u krvi. Less
Abstract (english)
Introduction: Lymphocytes belong to the leukocyte lineage and include three subpopulations, T and B lymphocytes and NK cells. T and B lymphocytes belong to acquired immunity while NK cells have the characteristics of innate immunity. Morphologically, the cell populations mentioned above do not differ. NK cells do not need matching with MHC molecules for cytolytic activity against extraneous antigen. Leukocyte differentiation antigens determine cell lineage and stage of maturity, and are... More called CD antigens. NK cells are CD3- and CD16+56. By using the methods of immunofluorescence, CD antigens are marked with anti-CD antibodies to which fluorochromes are bound. Flow cytometry is a diagnostic method that allows immunophenotyping of cells based on cell markers (CD markers). By flow cytometry, with the use of immunophenotyping, it is possible to determine NK cells in peripheral blood, ie the proportion within lymphocytes and the absolute number in blood. The flow cytometry results are most often presented in the form of cytograms. Objective: The purpose of this study was to determine the absolute number of NK cells from a peripheral blood sample using the flow cytometry methods Materials and methods: A sample of one patient admitted to the Department of Medical Laboratory Diagnostics of the Clinical Hospital Center Split (KBC Split) was processed. After the analysis on a hematology counter, there was processing of the sample by staining with fluorescently labeled antibodies to CD45, CD3 and CD16+56, followed by lysis and washing of erythrocytes. The processed sample was analyzed on a FACS Calibur flow cytometer. Results: By the methods of data analysis obtained by flow cytometry from three selected appropriate cytograms, percentage of lymphocytes within leukocytes and the percentage of NK cells within the lymphocyte population were obtained. The absolute number of NK cells was obtained by mathematical calculation using the number of leukocyte measured on the Sysmex hematology analyser XN-1000. Conclusion: Immunophenotyping method should be used to determine NK cells in peripheral blood. The absolute number and the percentage of NK cells determined in the analysed sample were within reference interval. Less
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:176:044037
Study programmeTitle: Medical Laboratory Diagnostics (university/undergraduate)
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni prvostupnik/prvostupnica (baccalaureus/baccalaurea) medicinska laboratorijska dijagnostika (sveučilišni prvostupnik/prvostupnica (baccalaureus/baccalaurea) medicinska laboratorijska dijagnostika)
Type of resourceText
File originBorn digital
Access conditionsAccess restricted to students and staff of home institution
Terms of useLicence In copyright icon
Created on2020-08-04 12:18:33
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