AIM: The main aim of this study was to investigate the impact of various factors on the attitudes and motivation of nurses towards continuing education in Croatia. METHODS: The research was conducted through an online questionnaire which consisted of three parts. The first part of the questionnaire includes the demographic determinants of the participants. The second part of the questionnaire refers to the assessment of motivation according to work preference. The third part of the questionnaire refers to the participant’s self-assessment and attitudes about possible obstacles to continuing education. The research included nurses employed in the profession with completed high school or higher education degree. RESULTS: Our results show that the highest level of motivation (intrinsic and extrinsic) is present in participants who have already enrolled in nursing studies, but also in those with a higher level of education. According to WPI score, there were no statistically significant difference observed between motivation and years of service. The analysis of the level of motivation between younger (<35 years) and older (> 35 years) participants did not reveal a statistically significant difference for intrinsic (P 0.912) and extrinsic motivation (P 0.622). But there is a visible difference for all questions of self-assessment of attitudes in which younger participants indicate a better salary, the possibility of learning something new, as reasons for continuing education. On the other hand, participants of this research (> 35 years old) felt that they will not be recognized with a higher level of education. Participants who are employed in hospital health care institutions show a lower level of intrinsic and extrinsic motivation towards continuing education compared to respondents employed in other health care institutions. CONCLUSION: Considering the obtained results, we can conclude that the level of motivation and more positive attitudes for continuing education are more present in younger participants (<35 years), but also in those participants who have previously completed higher education.