THE AIM: The aim of this thesis is to compare ELISA and CLIA method for the determination of autoantibodies to tissue transglutaminase type IgA (anti-tTG IgA) and autoantibodies to deamidated gliadine peptide type IgG (anti-DPG IgG) and with the comparison of the obtained results determine whether there are significant differences in the results. METHODS: For the purpose of introducing new, fully automated chemiluminescence assays for the determination of anti-tTG IgA and anti-DPG IgG antibodies (ZENIT RA t-TG IgA and ZENIT RA Deamidated Gliadin IgG, A. Menarini Diagnostics) on the IDS ISYS analyzer was conducted a verification of the methods which included series precision estimation, inter assay precision estimation and comparison with other methods (qualitative ELISA assays QUANTA Lite anti-tTG IgA and QUANTA Lite GliadinIgG II, INOVA, San Diego, CA). Precision in the series was determined by analyzing three poorly positive and four highly positive samples in triplicate. Inter assay precision was determined by analyzing one poorly positive and two highly positive samples during three days. The comparison of methods was conducted on 30 pacient samples of anti-tTG IgA and 28 samples for anti-DPG IgG. RESULTS: The coefficient of variation (CV) for precision in the anti-tTG IgA series ranged from 0.57 to 6.47%, and for inter assay precision from 1.53 to 4.87%. CV for precision in the anti-DPG IgG series was from 0.31 to 6.35% and for a median of 1.36 to 3.04%. Comparison of the methods showed concordant results for anti-tTG IgA (2/30, deviation 6.67%) and anti-DPG IgG (1/28; 3.57% deviation) CONCLUSION: The tested assays showed satisfying precision. Differences in the results of the parallel analysis were noted at the limit values. The comparison of the methods met the defined eligibility criteria (<10% deviations). The anti-tTG IgA antibody reagent provides parallel specific and total IgA quantification, which allows the recognition of those patients to whom specific anti-IgG classes must be determined. The stability of the test and the reaction conditions allows the use of saved calibration curve. With satisfying verification results, the introduction of new chemiluminescence methods for the determination of anti-tTG IgA and anti-DPG IgG shortens TAT since antibodies, unlike ELISA method, can be determined individually.