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undergraduate thesis
Usporedba dvije metode za određivanje autoantitijela na tkivnu transglutaminazu tipa IgA i autoantitijela na deamidirane glijadinske peptide tipa IgG
2017. urn:nbn:hr:176:291401
Jurić, Ana
University of Split
University Department of Health Studies

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Jurić, A. (2017). Usporedba dvije metode za određivanje autoantitijela na tkivnu transglutaminazu tipa IgA i autoantitijela na deamidirane glijadinske peptide tipa IgG (Undergraduate thesis). Split: University of Split. Retrieved from https://urn.nsk.hr/urn:nbn:hr:176:291401

Jurić, Ana. "Usporedba dvije metode za određivanje autoantitijela na tkivnu transglutaminazu tipa IgA i autoantitijela na deamidirane glijadinske peptide tipa IgG." Undergraduate thesis, University of Split, 2017. https://urn.nsk.hr/urn:nbn:hr:176:291401

Jurić, Ana. "Usporedba dvije metode za određivanje autoantitijela na tkivnu transglutaminazu tipa IgA i autoantitijela na deamidirane glijadinske peptide tipa IgG." Undergraduate thesis, University of Split, 2017. https://urn.nsk.hr/urn:nbn:hr:176:291401

Jurić, A. (2017). 'Usporedba dvije metode za određivanje autoantitijela na tkivnu transglutaminazu tipa IgA i autoantitijela na deamidirane glijadinske peptide tipa IgG', Undergraduate thesis, University of Split, accessed 01 April 2025, https://urn.nsk.hr/urn:nbn:hr:176:291401

Jurić A. Usporedba dvije metode za određivanje autoantitijela na tkivnu transglutaminazu tipa IgA i autoantitijela na deamidirane glijadinske peptide tipa IgG [Undergraduate thesis]. Split: University of Split; 2017 [cited 2025 April 01] Available at: https://urn.nsk.hr/urn:nbn:hr:176:291401

A. Jurić, "Usporedba dvije metode za određivanje autoantitijela na tkivnu transglutaminazu tipa IgA i autoantitijela na deamidirane glijadinske peptide tipa IgG", Undergraduate thesis, University of Split, Split, 2017. Available at: https://urn.nsk.hr/urn:nbn:hr:176:291401

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Metadata
TitleUsporedba dvije metode za određivanje autoantitijela na tkivnu transglutaminazu tipa IgA i autoantitijela na deamidirane glijadinske peptide tipa IgG
Title (english)COMPARISON OF TWO METHODS FOR DETERMINING AUTOANTIBODIES TO TISSUE TRANSGLUTAMINASE TYPE IgA AND AUTOANTIBODIES ON DEAMIDATED GLIADIN PEPTIDE TYPE IgG
AuthorAna Jurić
MentorLeida Tandara (mentor)
Committee memberDaniela Šupe-Domić (predsjednik povjerenstva)
Committee memberFrane Mihanović (član povjerenstva)
Committee memberLeida Tandara (član povjerenstva)
GranterUniversity of Split
(University Department of Health Studies)
Split
Defense date and country2017-09-28, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Clinical Medical Sciences
Abstract
CILJ: Cilj rada je usporediti ELISA i CLIA metodu za određivanje autoantitijela na tkivnu transglutaminazu tipa IgA (anti-tTG IgA) i autoantitijela na deamidirane glijadinske peptide tipa IgG (anti-DPG IgG) te usporedbom dobivenih rezultata utvrditi postoje li značajne razlike u rezultatima. METODE: U svrhu uvođenja novih, potpuno automatiziranih kemiluminiscentnih testova za određivanje anti-tTG IgA i anti-DPG IgG antitijela (ZENIT RA t-TG IgA i ZENIT RA Deamidated Gliadin IgG, A.... More Menarini Diagnostics) na analizatoru IDS ISYS provedena je verifikacija metoda koja je obuhvatila procjenu preciznosti u seriji, procjenu međupreciznosti i usporedbu s drugim metodama (kvalitativnim ELISA testovima QUANTA Lite anti-tTG IgA i QUANTA Lite Gliadin IgG II; INOVA, San Diego, CA). Preciznost u seriji određena je analiziranjem tri slabo pozitivna i četiri jako pozitivna uzorka u triplikatu. Međupreciznost je određena analiziranjem jednog slabo pozitivnog i dva jako pozitivna uzorka tijekom tri dana. Usporedba metoda provedena je na 30 uzoraka pacijenata za anti-tTG IgA i 28 uzorka za anti-DPG IgG. REZULTATI: KV za preciznost u seriji za anti-tTG IgA kretao se od 0,57 do 6,47%, a za međupreciznost 1,53 do 4,87%. KV za preciznost u seriji za anti-DPG IgG bio je 0,31 do 6,35%, a za međupreciznost od 1,36 do 3,04%. Usporedba metoda pokazala je zadovoljavajuće slaganje rezultata za anti-tTG IgA (2/30; odstupanje 6,67%) i anti-DPG IgG (1/28; odstupanje 3,57%). ZAKLJUČAK: Ispitivani testovi su pokazali zadovoljavajuću preciznost. Razlike u rezultatima usporedne analize zabilježene su kod graničnih vrijednosti. Usporedba metoda zadovoljila je definirani kriterij prihvatljivosti (<10% odstupanja). Reagens za određivanje anti-tTG IgA omogućava istovremenu kvantifikaciju specifičnog i ukupnog IgA što omogućava prepoznavanje onih pacijenata kojima je nužno odrediti specifično antitijelo IgG klase. Stabilnost testa i reakcijskih uvjeta omogućava korištenje pohranjene kalibracijske krivulje. Uz zadovoljavajuće rezultate verifikacije, uvođenje nove kemiluminiscentne metode za određivanje anti-tTG IgA i anti-DPG IgG skraćuje vrijeme do izdavanja nalaza budući da se antitijela, za razliku od ELISA metode, mogu određivati pojedinačno. Less
Abstract (english)
THE AIM: The aim of this thesis is to compare ELISA and CLIA method for the determination of autoantibodies to tissue transglutaminase type IgA (anti-tTG IgA) and autoantibodies to deamidated gliadine peptide type IgG (anti-DPG IgG) and with the comparison of the obtained results determine whether there are significant differences in the results. METHODS: For the purpose of introducing new, fully automated chemiluminescence assays for the determination of anti-tTG IgA and anti-DPG... More IgG antibodies (ZENIT RA t-TG IgA and ZENIT RA Deamidated Gliadin IgG, A. Menarini Diagnostics) on the IDS ISYS analyzer was conducted a verification of the methods which included series precision estimation, inter assay precision estimation and comparison with other methods (qualitative ELISA assays QUANTA Lite anti-tTG IgA and QUANTA Lite GliadinIgG II, INOVA, San Diego, CA). Precision in the series was determined by analyzing three poorly positive and four highly positive samples in triplicate. Inter assay precision was determined by analyzing one poorly positive and two highly positive samples during three days. The comparison of methods was conducted on 30 pacient samples of anti-tTG IgA and 28 samples for anti-DPG IgG. RESULTS: The coefficient of variation (CV) for precision in the anti-tTG IgA series ranged from 0.57 to 6.47%, and for inter assay precision from 1.53 to 4.87%. CV for precision in the anti-DPG IgG series was from 0.31 to 6.35% and for a median of 1.36 to 3.04%. Comparison of the methods showed concordant results for anti-tTG IgA (2/30, deviation 6.67%) and anti-DPG IgG (1/28; 3.57% deviation) CONCLUSION: The tested assays showed satisfying precision. Differences in the results of the parallel analysis were noted at the limit values. The comparison of the methods met the defined eligibility criteria (<10% deviations). The anti-tTG IgA antibody reagent provides parallel specific and total IgA quantification, which allows the recognition of those patients to whom specific anti-IgG classes must be determined. The stability of the test and the reaction conditions allows the use of saved calibration curve. With satisfying verification results, the introduction of new chemiluminescence methods for the determination of anti-tTG IgA and anti-DPG IgG shortens TAT since antibodies, unlike ELISA method, can be determined individually. Less
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:176:291401
Study programmeTitle: Medical Laboratory Diagnostics (university/undergraduate)
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni prvostupnik/prvostupnica (baccalaureus/baccalaurea) medicinska laboratorijska dijagnostika (sveučilišni prvostupnik/prvostupnica (baccalaureus/baccalaurea) medicinska laboratorijska dijagnostika)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2020-05-06 07:32:02
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