Objectives: The aim of this study was to determine the knowledge , skills and attitudes about hand hygiene in secondary education of future health professionals. Participants and methodology: The subjects were students of first, second, third, fourth and fifth grade of Medical School in Split, which the school year 2015/2016. attend educational programs: a nurse responsible for general care - nurse of general care, medical laboratory technician, pharmacy technician, physiotherapy technician, sanitary technician, dental assistant / assistant, dental technician / technician. The study was cross-sectional. Conducted three - part questionnaire in the Health School of Split through the month of September 2015. It is anticipated that the use of a three - part questionnaire. The first part contains questions from cognitive areas (knowledge and understanding) on hand hygiene as a prerequisite for application in practice. The other part contains questions from the psychomotor areas (traditional skills of hand hygiene) with the aim of precision. The third part contains questions from the affective domain (attitudes and beliefs about hand hygiene) with a view to adopting values. The survey is in the annexes. Data were statistically analyzed and presented in tables and graphs. For the purpose of proving / disproving hypotheses set up in this thesis used the Chi-square test. The level of significance was set at p <0.05. Results: There is a statistically significant difference in knowledge, skills and attitudes about hand hygiene among students initial and final grades, and the students' final grades Medical School in Split school year 2015/2016. Final year students significantly more give correct answers to questions from the group claims knowledge, significantly more are considered to have the necessary skills and attitudes about hand hygiene as opposed to students starting grade. Among students in their final responses (five) class occupations nurse technician general direction and pupils in the last (fourth) class of other professions there is a statistically significant difference in the responses from the group assertion skills with final year students of other professions considered to have less necessary skills on hand hygiene. Conclusion Hand hygiene is considered the most effective single method of prevention of infections that occur during provision of health care. It is extremely important in preventing the transmission of harmful microorganisms by human hands to food, work surfaces and other people. Hygienic hand disinfection is carried out hygienic washing and rubbing his hand, which removes most transient microorganisms on the hands. It's very simple habit that requires minimal training and does not require special equipment, so it is necessary for students Medical schools since the initial class convey knowledge of proper hand hygiene in order as soon as possible acquire the necessary skills and attitudes.