Title Utjecaj inhibitora fosfolipaze C na rast i preživljenje stanica karcinoma prostate Title (english) INFLUENCE OF FOSFOLIPASE C INHIBITORS ON GROWTH AND SURVIVAL PROSTATE CANCER CELLS Author Antea Medak Mentor Nikolina Režić Mužinić (mentor)Committee member Nikolina Režić Mužinić (član povjerenstva)Committee member Ivana Franić (član povjerenstva)Committee member Vedrana Čikeš Čulić (član povjerenstva)Granter University of Split Defense date and country 2018-07-12, Croatia Scientific / art field, BIOMEDICINE AND HEALTHCARE Abstract Uvod:
Karcinom prostate jedan je od najčešćih karcinoma u muškoj populaciji, stoga su zbog njegovog učestalog pojavljivanja predstavljena brojna istraživanja koja nastoje poboljšati, skratiti i omogućiti što kvalitetnije liječenje pacijenta. Progresija tumora može biti potaknuta od strane matičnih stanica karcinoma. Tijekom ovog istraživanja procijenjen je metabolizam stanica pomoću MTT testa nakon primjene novosintetiziranog spoja samostalno ili u kombinaciji s paclitaxel-om. Također im je određena rana i kasna apoptoza pomoću Annexin-V i PI bojenja. Upravo se razaranje matičnih stanica karcinoma prostate smatra obećavajućim ciljem liječenja karcinoma prostate kako bi se poboljšao ishod u pacijenata s naprednim stadijem bolesti.
Cilj:
Cilj ovog rada bio je utvrditi postotak vijabilnosti stanica karcinoma prostate Du-145 nakon tretmana s novosintetiziranim spojem (3-Amino-5okso-N-naftil-5,6,7,8-tetrahidrotieno[2,3-b]kinolin-2-karboksamid) - inhibitorom fosfolipaze C.
Materijali i metode:
Stanice Du-145 su inkubirane s novosintetiziranim spojem ili u kombinaciji s paclitaxel-om. Stanice su uzgajane u specifičnim uvjetima, a vijabilnost stanica određena je pomoću MTT testa. Vrsta stanične smrti procijenjena je nakon 48 sati pomoću Annexin-V-FITC testa i propidij jodida. Podaci su prikupljeni pomoću BD Accuri C6 citometra i analizirani korištenjem FlowLogic Softvera.
Rezultati:
Rezultati koji su dobiveni nakon tretmana s novosintetiziranim spojem ovisili su o koncentracijama i vremenskom razdoblju tretmana. Nakon 72h, niže koncentracije novosintetiziranog spoja, u iznosu od 1 μM pokazivale su značajnu citotoksičnost u odnosu na netretirane stanice. Također, rezultati su pokazali značajno smanjenje preživjelih stanica nakon korištenja kombinacije novosintetiziranog spoja (0,5 μM) i paclitaxel-a (25 μM) nakon 48 i 72 sata.
25
Zaključak:
Ustanovljeno je kako novosintetizirani spoj, 3-amino-5-okso-N-naftil-5,6,7,8-tetrahidrotieno[2,3-b] kinolin-2-karboksamid, citotoksičan za stanice karcinoma prostate. Također vrlo važno je spomenuti kako su prilikom korištenja novositetiziranog spoja presudne koncentracije tog istog spoja te vremenski period u kojem je spoj korišten.
Abstract (english) Introduction:
Prostate cancer is one of the most common cancer in the male population, therefore, due to its frequent occurrence, a number of researches are being carried out that seek to improve, shorten and enable the best quality of treatment for the patient. Tumor progression may be triggered by cancer stem cells (CSCs). During this study, cell metabolism was evaluated using the MTT assay after application of the newly synthesized compound alone or in combination with paclitaxel. Early and late apoptosis was also determined by using Annexin-V and PI staining. Precisely, this destruction of prostate cancer steam cells is considered as promising goal of prostate cancer treatment to improve outcome in patients with advanced stage of disease.
Aim:
The aim of this paper was to determine the cell viability of prostate cancer cells Du-145 after treatment with the newly synthesized compound (3-Amino-5-oxo-N-naphthyl-5,6,7,8-tetrahydrothieno [2,3-b] quinoline-2-carboxamide) inhibitor of phospholipase C.
Materials and methods:
Du-145 cells were incubated with either only newly synthesized compound or in combination with paclitaxel. The cells were cultured under specific conditions, and cell viability was determined by the MTT test. Cell death was evaluated after 48 hours using Annexin-V-FITC test and propidium iodide. The data were collected using the BD Accuri C6 cytometer and analyzed using FlowLogic Software.
The results:
The results obtained after treatment with the newly synthesized compound depended on the concentrations and the time of treatment. After 72 hours, lower concentration of the novosynthetic compound, in the amount of 1 μM showed significant cytotoxicity in comparison to untreated cells. Also, the results showed a significant reduction in surviving cells after using the combination of newly synthesized compound (0,5 μM) and paclitaxel (25 μM) after 48 and 72 hours.
27
Conclusion:
It has been found that newly synthesized compound, 3-amino-5-oxo-N-naphthyl-5,6,7,8-tetrahydrothieno [2,3-b] quinoline-2-carboxamide is cytotoxic to prostate cancer cells. Also, it is very important to mention while using the newly synthesized compound, the critical is concentration of the same compound and the time period in which the compound is used.
Keywords
Karcinom prostate
matične stanice karcinoma
novosintetizirani antitumorski spoj (ključne riječi unio urednik)
Keywords (english)
prostate cancer
cancer stem cells
newly synthesized anticancer agent (ključne riječi unio urednik)
Language croatian URN:NBN urn:nbn:hr:176:018699 Study programme Title: Medical Laboratory Diagnostics (university/undergraduate) Type of resource Text File origin Born digital Access conditions Open access Terms of use Created on 2020-05-25 10:14:30
