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undergraduate thesis
Molekularna dijagnostika i ciljano liječenje karcinoma debelog crijeva
2023. urn:nbn:hr:176:199179
Primorac, Korina
University of Split
University Department of Health Studies

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Primorac, K. (2023). Molekularna dijagnostika i ciljano liječenje karcinoma debelog crijeva (Undergraduate thesis). Split: University of Split. Retrieved from https://urn.nsk.hr/urn:nbn:hr:176:199179

Primorac, Korina. "Molekularna dijagnostika i ciljano liječenje karcinoma debelog crijeva." Undergraduate thesis, University of Split, 2023. https://urn.nsk.hr/urn:nbn:hr:176:199179

Primorac, Korina. "Molekularna dijagnostika i ciljano liječenje karcinoma debelog crijeva." Undergraduate thesis, University of Split, 2023. https://urn.nsk.hr/urn:nbn:hr:176:199179

Primorac, K. (2023). 'Molekularna dijagnostika i ciljano liječenje karcinoma debelog crijeva', Undergraduate thesis, University of Split, accessed 23 April 2024, https://urn.nsk.hr/urn:nbn:hr:176:199179

Primorac K. Molekularna dijagnostika i ciljano liječenje karcinoma debelog crijeva [Undergraduate thesis]. Split: University of Split; 2023 [cited 2024 April 23] Available at: https://urn.nsk.hr/urn:nbn:hr:176:199179

K. Primorac, "Molekularna dijagnostika i ciljano liječenje karcinoma debelog crijeva", Undergraduate thesis, University of Split, Split, 2023. Available at: https://urn.nsk.hr/urn:nbn:hr:176:199179

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Metadata
TitleMolekularna dijagnostika i ciljano liječenje karcinoma debelog crijeva
Title (english)Molecular diagnostics and targeted treatment of colon cancer
AuthorKorina Primorac
MentorSendi Kuret (mentor)
Committee memberDavorka Sutlović (predsjednik povjerenstva)
Committee memberEsma Čečuk-Jeličić (član povjerenstva)
Committee memberSendi Kuret (član povjerenstva)
GranterUniversity of Split
(University Department of Health Studies)
Split
Defense date and country2023-07-17, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Clinical Medical Sciences
Abstract
Cilj: Cilj ovog rada je prikazati molekularno testiranje kojim se utvrđuje status gena KRAS, NRAS i BRAF kao prediktivnih biomarkera kod pacijenata s karcinomom debelog crijeva radi primjene ciljane terapije u njihovu liječenju. Materijal i metode: Materijal koji se koristi za analizu je tumorsko tkivo pacijenta. Opisan je postupak izolacije DNA, mjerenja koncentracije DNA te umnožavanje DNA uz pomoć komercijalno dostupnih kitova. Rezultati: Rezultati koji se dobiju metodom lančane reakcije polimerazom u stvarnom vremenu (RT-PCR) obrađuju se softwerski, a potom se analiziraju postojeće mutacije u uzorku pacijenta. Zaključak: U ovom radu prikazana je važnost molekularnog testiranja gena KRAS, NRAS i BRAF kod pacijenata s kolorektalnim karcinomom u svrhu primjene ciljane terapije. Kako bi se provela analiza i utvrdila prisutnost mutacija, potrebno je iz uzorka tumorskog tkiva izolirati DNA, odrediti njenu koncentraciju, a zatim ju umnožiti.
Abstract (english)
Objectives: The aim of this paper is to present molecular testing that determines the status of KRAS, NRAS and BRAF genes as predictive biomarkers in patients with colon cancer for the purpose of applying targeted therapy in their treatment. Material and methods: The material used for analysis is the patient's tumor tissue. The procedure for DNA isolation, measurement of DNA concentration and DNA amplification with the help of commercially available kits is described. Results: The results obtained by the real-time polymerase chain reaction (RT-PCR) method are processed by software, and then the existing mutations in the patient's sample are analysed. Conclusion: This paper shows the importance of molecular testing of KRAS, NRAS and BRAF genes in patients with colorectal cancer for the purpose of applying targeted therapy. In order to carry out the analysis and determine the presence of mutations, it is necessary to isolate DNA from the tumor tissue sample, determine its concentration, and then multiply it.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:176:199179
Study programmeTitle: Medical Laboratory Diagnostics (university/undergraduate)
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni prvostupnik/ prvostupnica (baccalaureus/ baccalaurea) medicinske laboratorijske dijagnostike (sveučilišni prvostupnik/ prvostupnica (baccalaureus/ baccalaurea) medicinske laboratorijske dijagnostike)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2023-07-17 09:20:57